Harvard Medical School, Massachusetts Eye & Ear Infirmary

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Lama Mulki^*1,2, J. Harry Sweigard^*1, Kip M. Connor¹

¹Angiogenesis Laboratory, Department of Ophthalmology, Harvard Medical School, Massachusetts Eye & Ear Infirmary, ²St. Elizabeth's Medical Center

Here, we present a protocol that allows the investigator to assess leukocyte recruitment dynamics ex vivo by connecting a chamber coated with endothelial-derived adhesion molecules to the circulatory system of a mouse. This method offers significant advantages since it allows for leukocyte assessment under relative biological conditions.